polyclonal rabbit cc3 antibody  (Cell Signaling Technology Inc)

Journal: Frontiers in Nutrition

Article Title: Immunomodulatory effects of short-chain fatty acids and immune-supporting nutrients on slice cultures of head and neck tumors

doi: 10.3389/fnut.2026.1731077

Figure Lengend Snippet: Means of CC3 intensity in four different culturing conditions (ns, not significant; ** p < 0.01). Means were compared by Kruskal-Wallis test.

Article Snippet: Immunostaining for cleaved caspase-3 (CC3) was conducted on a Ventana Discovery Ultra immunostainer (Ventana Roche, USA) using a polyclonal rabbit CC3 antibody (diluted at 1:400, #9661, Cell Signalling Technology).

Techniques:

Journal: Frontiers in Nutrition

Article Title: Immunomodulatory effects of short-chain fatty acids and immune-supporting nutrients on slice cultures of head and neck tumors

doi: 10.3389/fnut.2026.1731077

Figure Lengend Snippet: EMM of relative CC3 intensity in 9 patients. Patient 6 and patient 9 (highest intensities) significantly differed from all other patients (Error bars: SEM; ** p < 0.01; *** p < 0.001).

Article Snippet: Immunostaining for cleaved caspase-3 (CC3) was conducted on a Ventana Discovery Ultra immunostainer (Ventana Roche, USA) using a polyclonal rabbit CC3 antibody (diluted at 1:400, #9661, Cell Signalling Technology).

Techniques:

Journal: Frontiers in Nutrition

Article Title: Immunomodulatory effects of short-chain fatty acids and immune-supporting nutrients on slice cultures of head and neck tumors

doi: 10.3389/fnut.2026.1731077

Figure Lengend Snippet: Estimated marginal means (EMMs) of relative CC3 intensity across treatment conditions control, immunonutrition (IN), short-chain fatty acids (SCFAs), and combined IN + SCFAs (Error bars: SEM; ** p < 0.01, and *** p < 0.001).

Article Snippet: Immunostaining for cleaved caspase-3 (CC3) was conducted on a Ventana Discovery Ultra immunostainer (Ventana Roche, USA) using a polyclonal rabbit CC3 antibody (diluted at 1:400, #9661, Cell Signalling Technology).

Techniques: Control

Journal: Frontiers in Nutrition

Article Title: Immunomodulatory effects of short-chain fatty acids and immune-supporting nutrients on slice cultures of head and neck tumors

doi: 10.3389/fnut.2026.1731077

Figure Lengend Snippet: CC3 staining comparing control condition with immunonutrition and immunonutrition combined with SCFAs (blue: CC3 negative cells; brown: CC3 positive cells). (A1) Control condition patient 3; CC3 positive cells in stroma and tumor regions. (A2) Immunonutrition patient 3; lower amount of CC3 positive cells compared to control. (A3) SCFA condition patient 3; CC3 positive cells mostly in tumor regions. (B1) Control condition patient 6; CC3 positive cells in stroma and tumor regions. (B2) Immunonutrition patient 6; lower amount of CC3 positive cells compared to control. (B3) Immunonutrition + SCFAs patient 6; CC3 positive cells mostly in tumor regions (Scale bars: 50 μm).

Article Snippet: Immunostaining for cleaved caspase-3 (CC3) was conducted on a Ventana Discovery Ultra immunostainer (Ventana Roche, USA) using a polyclonal rabbit CC3 antibody (diluted at 1:400, #9661, Cell Signalling Technology).

Techniques: Staining, Control

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Diverse Misfolding Mutant Digestive Enzymes Cause Chronic Pancreatitis Through Common Pathways

doi: 10.1016/j.jcmgh.2025.101638

Figure Lengend Snippet: Immunohistochemistry staining showing increased DNA damage and cell cycle markers in the pancreas of Cpa1 N256K and Pnlip T221M mice. The mice analyzed are WT, Cpa1 N256K, and Pnlip T221M homozygous animals. Representative immunohistochemistry images showing increased DNA damage, cell proliferation, and apoptosis in the pancreas at 6 months of age. The markers include: phospho-histone H2A.X (p-γH2A.X), p-HH3, Ki67, p53, p21, and CC3. Scale bar is 100 μm. The quantification graphs are presented on the right for each stain. Data quantification was carried out as described in the Methods section. The quantification data are presented as mean values with SD, with 20 fields randomly selected and analyzed for each group. One-way ANOVA with multiple comparisons was performed for each stain. Symbols ∗, ∗∗, ∗∗∗, and ∗∗∗∗ represent P < .05, .01, .001, and .0001, respectively. P < .05 indicates a statistically significant difference between groups, and ns means not statistically significant.

Article Snippet: The primary antibodies used in this study were as follows: a rabbit monoclonal F4/80 antibody (1:100; 70076, Cell Signaling Technology); a rabbit monoclonal CD3 antibody (1:100; 16669, Abcam); a rabbit polyclonal CD45 antibody (1:2000; 10558, Abcam); a rabbit polyclonal myeloperoxidase (MPO) antibody (1:50; 9535, Abcam); a rabbit polyclonal p53 antibody (1:200; NCL-L-p53-CM5p, Leica Biosystems); a rabbit monoclonal p21 antibody (1:500; 188224, Abcam); a rabbit polyclonal Ki67 antibody (1:800; 15580, Abcam); a rabbit polyclonal phospho-histone H3 Ser10 (p-HH3) antibody (1:1200; 5176, Abcam); a rabbit monoclonal phospho-histone H2A.X Ser139 (p-γH2A.X) antibody (1:800; 9718, Cell Signaling Technology); a rabbit polyclonal CC3 antibody (1:300; 9661, Cell Signaling Technology).

Techniques: Immunohistochemistry, Staining

Journal: Molecular Cancer

Article Title: The anti-diabetic PPARγ agonist Pioglitazone inhibits cell proliferation and induces metabolic reprogramming in prostate cancer

doi: 10.1186/s12943-025-02320-y

Figure Lengend Snippet: Pioglitazone suppresses cell migration of PCa cells and growth of metastatic PC3 xenograft tumors. a Relative wound density resulting from scratch wound assay of 22RV1 (left) and PC3 (right) cells for 24 hours treatment with each PPAR agonist (100 µM, vehicle control = 0.2 % DMSO). One-way ANOVA was used to test for significance (ns = not significant p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001). Data represent the means ± SD of biological triplicates. b Workflow scheme of the mouse xenograft experiment of PC3 cells in male NSG mice. c Tumor volume throughout the 14 days of treatment with Tesaglitazar (0.4 mg/kg) and Pioglitazone (10 mg/kg) or vehicle control (20 % hydroxypropyl-beta cyclodextrin) (n = 6). Significance was evaluated by two-way ANOVA (ns = not significant p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001). d Representative images of immunohistochemistry (IHC) evaluation of Ki67, CC3, phospho AMPKα, and phospho mTOR in xenograft tumors after treatment with Tesaglitazar, Pioglitazone or vehicle control (20x magnification, scale bar = 50 µm). e IHC quantifications of phospho AMPKα, and phospho mTOR of Tesaglitazar and Pioglitazone treated xenograft tumors (n = 4). Significance was evaluated by one-way ANOVA (ns = not significant p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001). f, g Kaplan-Meier analysis showing BCR-free survival of age-matched non-diabetic (n = 20) and diabetic PCa patients (n = 47) ( f ), as well as diabetic patients treated with SGLT2 inhibitors (n = 15), Metformin (n = 17), PPAR agonists (n = 3), Insulin (n = 4) and DDP4 plus Metformin (n = 14) ( g ). Log-rank (Mantel-Cox) test was used to test for significance ( p ≤ 0.05)

Article Snippet: Rabbit polyclonal CC3 (Asp175) , Cell Signaling , #9661.

Techniques: Migration, Scratch Wound Assay Assay, Control, Immunohistochemistry

Journal: iScience

Article Title: A human-specific, concerted repression of microcephaly genes contributes to radiation-induced growth defects in cortical organoids

doi: 10.1016/j.isci.2025.111853

Figure Lengend Snippet:

Article Snippet: Rabbit Polyclonal anti-CC3 (Asp175) , Cell Signaling , Cat# 9661; RRID: AB_2341188.

Techniques: Recombinant, Selection, Modification, Transfection, Knock-Out, Lysis, TUNEL Assay, In Situ, Reverse Transcription, Irradiation, Control, Software, Functional Assay, Gene Expression

Journal: Cell reports

Article Title: The RNA-binding protein EIF4A3 promotes axon development by direct control of the cytoskeleton

doi: 10.1016/j.celrep.2024.114666

Figure Lengend Snippet: (A) Left: canonical RNA regulation by the exon junction complex (EJC) composed of core components EIF4A3, RBM8A, and MAGOH. Right: the question addressed in this study. How do EJC components control neuronal development?. (B) Coronal section from a control ( Eif4a3 lox/lox ) E17.5 mouse cortex, stained with L1CAM and Hoechst. (C) Higher-magnification images from the region indicated by the dashed box in (B), stained with L1CAM and CC3. White bracket, cortical axonal tract thickness. Arrow, region with apoptotic nuclei. (D) Fold change of L1CAM+ axonal thickness in E17.5 Magoh, Rbm8a, and Eif4a3 cHet and cKO cortices relative to control ( Magoh lox/lox , Rbm8a lox/lox and Eif4a3 lox/lox , respectively). ** p = 0.0065, one-way ANOVA (Dunnett’s multiple-comparisons test), n = 3–6 embryos. (E) CC3+ cell density in E17.5 Magoh, Rbm8a, and Eif4a3 cHet and cKO relative to control ( Magoh lox/lox , Rbm8a lox/lox , and Eif4a3 lox/lox , respectively). ** p < 0.01, *** p < 0.001, one-way ANOVA (Dunnett’s multiple-comparisons test), n = 3 embryos. (F) Coronal sections of control ( Eif4a3 lox/lox ; p53 lox/lox ) and Eif4a3; p53 dcKO E17.5 mouse cortices, stained with L1CAM, CC3, and Hoechst. White bracket, cortical axonal tract thickness. (G) Fold change of L1CAM+ cortical axonal thickness in E17.5 Eif4a3, p53 dcHet, and dcKO relative to control ( Eif4a3 lox/lox ; p53 lox/lox ). ** p = 0.0099, one-way ANOVA (Dunnett’s multiple-comparisons test), n = 4 embryos. (H) Fold change of L1CAM+ fibers/cortical thickness ratio from E17.5 Eif4a3 cKO relative to control ( Eif4a3 lox/lox ) and on a p53 background. * p = 0.0491, *** p < 0.001, one-way ANOVA (Dunnett’s multiple-comparison test), n = 6 embryos. All graphs, mean + SD; ns, not significant. Scale bars: 500 μm (B), 100 μm (C), and 50 μm (F). See also and .

Article Snippet: Rabbit polyclonal anti-Cleaved Caspase-3 (CC3) (Asp175) , Cell Signaling Technology , Cat#9661; RRID:AB_2341188.

Techniques: Control, Staining, Comparison

Journal: Cell reports

Article Title: The RNA-binding protein EIF4A3 promotes axon development by direct control of the cytoskeleton

doi: 10.1016/j.celrep.2024.114666

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-Cleaved Caspase-3 (CC3) (Asp175) , Cell Signaling Technology , Cat#9661; RRID:AB_2341188.

Techniques: Virus, Recombinant, Electron Microscopy, Protease Inhibitor, Binding Assay, Spin Down Assay, Control, Plasmid Preparation, Software